Chromatography is a technique used to isolate the various components of a mixture and this application is very important in analysis of bio-molecules. The technique is used to separate and analyze the complex DNA sequences and other compounds, and also the concentration of the samples. Chromatography is a separation process involving two phases, stationary and mobile phase. Mixture to be analyzed is adsorbed in stationary phase and mobile phase is passed into it, eventually compounds of mixture get separated on basis of rate of adsorption and solubility. Both are physical properties. There are many types of chromatography used in the study of bio-molecules which range from DNA/RNA to recombinant proteins and antibodies. Column chromatography is one among the various types of chromatography.
In the process of column chromatography, a glass tube is filled with adsorbent, that is either alumina or silica gel up to a level of one thirds of the length of the glass tube. Then, it is soaked with selective solvent. Sometimes, column is filled with slurry, an amalgam of adsorbent and solvent. The column should not have any space, should be well packed. In this method, less polar compound will be eluted first. Because, less polar compound will be less adsorbed in polar stationary phase. Eventually, more polar compound will come out.
Sorbead India manufactures various grades of silica and alumina for chromatographic separations, especially for column chromatography. This endeavor measures the pore diameter using the BET method which guarantees the best and most accurate results during manufacturing process. Sorbead India offers products of various pore diameters for a range of different particle sizes, required for complex chromatographic separation processes. Column chromatography techniques help in extractions, synthesis of natural products of pharmaceutical ingredients and as a result these adsorbents are mainly used in chemical manufacturing units and pharma industry.
